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These probes were assayed in situ for their specificity to hybridise species of propionibacteria by observation using fluorescence microscopy and results were compared with the probe Pap446 previously designed for P. acidipropionici.
The observation using fluorescence microscope showed that characteristic punctate GFP-LC3 proteins accumulated both in the cells treated with rapamycin and in the cells infected with the influenza A virus, indicating that the autophagosomes were induced in both rapamycin-treated and influenza A virus-infected cells.
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The current work that was based on observations using fluorescence microscopy cannot also exclude the possibility that part of newly formed F-actin filaments were colocalized with existing microfilaments through actin filament-crosslinking proteins, e.g. fimbrin [ 8, 14].
Recent observations using fluorescence correlation spectroscopy have identified that both A32- and C11-like epitopes become exposed within minutes of incubation of HIV and target cells, further validating that these CD4i epitopes are opportune targets of the humoral immune response at extremely early stages of HIV infection (Mengistu et al., 2015).
Additional 20 30 μm thick cross sections of wounded stem were stained for tannins, callose and suberin observation, using a fluorescence microscope (excitation at 340 380 nm, and 410 450 nm barrier filters) (Olympus BX51).
It will also be possible to image actin remodelling with newer reporters of the actin cytoskeleton such as Lifeact (Riedl et al., 2008), and to carry out in situ observation of signalling pathways using fluorescence resonance energy transfer (FRET) (see Kamiyama and Chiba, 2009).
The efficiency of transfection was verified by observation of the fluorescence emitted by the Cy3-conjugated si-Control using fluorescence microscopy.
Xia, Z. & Liu, Y. Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes.
The fluorescence was read using fluorescence microscope.
The intensity of GFP fluorescence associated with the heart was then determined using fluorescence microscopy.
Proteasome activity was determined using fluorescence assays.
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