The observation that sister kinetochores are inherently biased to biorient on the mitotic spindle argues for this possibility (Indjeian and Murray 2007).
This indicates that TopoIV is a MukBEF cargo that would allow coordination of decatenation and segregation, consistent with the observation that sister cohesion is increased in Muk− cells (Danilova et al., 2007).
One of the sharpest observations my sister Johanna has ever made is that there is a difference between cooking and fixing food.
Observations that sister chromatid cohesion antagonizes CO formation by down-regulating both DSB catalysis and CO-generating inter-homolog DSB repair (e.g., Niu et al. 2005; Pan et al. 2011) suggest that the effect of chromosome size on pericentric recombination might be mediated by size-dependent loading of Rec8.
The observation that two sister species occur with different yet overlapping ranges in Madagascar raises obvious questions regarding the driving mechanisms behind their divergence.
In fact, observation of individual sister chromatids (cen2-GFP) [ 11, 13] showed that in the ndc80-21 mutwot, two cen2-GFP signals remained attached to the same pole instead of splitting into the two poles (wild-type, top).
Myxomycetes are rich in nuclear rDNA introns with a relatively large proportion containing HEGs and the possibility of an invading bacterial intron is supported by the recent observation of a sister intron to Dir.S956-1 in the myxomycete Diderma (SD Johansen, unpublished data).
This is most evident with the stark drop in observations when including sisters and sisters-in-law in the model.
The low transmissibility, if any, of M. abscessus from person to person is further supported by the observation of twin sisters in our series, only one of whom was colonized with M. abscessus.
This, however, seems to be at odds with observations that centromeric sister sequences split for significant distances during the chromosome bi-orientation process, long before separase is activated to remove cohesin from centromeres.
