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The GFP reporter could be readily seen after observation in live embryos, and was further amplified for embryo scoring and photographing by immuno-fluorescence staining of the GFP protein (see also below).
35 Teaching practical hands-on skills in short intensive reorientation sessions to our programme gastroenterologists and assessment of their performance and competency in key aspects of colonoscopy by direct observation in live case sessions by experienced colonoscopists were valuable in ensuring the high-quality colonoscopy services in this programme.
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"The method of measuring the skeleton and using it to estimate mass is based on the observation in living animals that, from a biomechanical standpoint, animals have the limbs they need -- no more and no less". Lacovara told The Washington Post.
We use computer simulations of mathematical models to compare our ideas regarding mechanisms with observations in live cells.
These observations in live spheroids were confirmed by immunohistochemical staining of fixed samples (Fig. 4B).
To summarize, the minimal biophysical model, which was synthesized from the very short-term observations in live imaging (up to 3 days), is capable of capturing the fate behavior of GFRα1+ spermatogonia from the short term (up to 20 days) to the long term (up to times comparable with the life span of mice).
Light microscopy allows for kinetic observations in living cells; in particular, modern fluorescence technology affords imaging of single fluorophores with high temporal resolution.
The direction of traction forces in the COT structure were consistent with observations in living cells [28], [37].
Observations in living cells have revealed that the fission of PGCs frequently coincides with mechanical pulling of carrier precursor from the TGN along microtubules.
H, P, and C MRS were used to quantify cellular metabolites in cell extracts following exposure to [1,2-C]-choline for 48 hours in order to further confirm the observations made in live cells and to monitor de novo synthesis of PtdCho and GPC.
Single molecule observation in large, living samples is problematic.
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