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The stainings were analyzed by an Olympus IX-70 inverted system microscope with IX-FLA observation attachment for fluorescence imaging.
ST14A cells transfected with GFP- and/or mRFP-tagged reporters were imaged directly in 6-well culture dishes using an Olympus IX70 inverted microscope equipped with an Olympus IX-FLA Inverted Reflected Light Fluorescence Observation attachment and RGB Mirror Cube filter wheel (Olympus).
The stainings were analysed by an Olympus IX-70 inverted system microscope with IX-FLA observation attachment for fluorescence imaging.
All fluorescence microscopy experiments were conducted on an Olympus IX70 inverted microscope equipped with an IX-FLA inverted reflected light fluorescence observation attachment.
Samples were analysed with an Olympus IX-70 inverted system microscope with IX-FLA observation attachment for fluorescence imaging, with a Zeiss LSM 780 confocal microscope or with a Leica DM6000 microscope (Leica Microsystems, Heidelberg, Germany).
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The above results were supported by scanning electron microscope (SEM) and confocal microscopy observation on attachment and growth of hBMSCs on PLA, PHBHHx and PHBVHHx, and by CCK-8 evaluation of cell proliferation.
The adhesion and proliferation behavior of OCT-1 osteoblast-like cells morphology on the pits- and islands-patterned surface were characterized by SEM observation, cell attachment efficiency measurement and MTT assay.
From FE-SEM observations, cell attachment and spreading of MG 63 cells showed significantly higher tendency for surfaces covered by HA coating and nanotubes.
This finding helps to explain one aspect of the long-standing observation that attachments under tension in vivo are stable.
Precise observation of cell attachment on G/Z was conducted.
This model is also consistent with the observation that the attachment site of ubiquitin on PCNA is apparently not crucial for activity, such that an in-frame fusion of ubiquitin to the amino terminus of PCNA supports TLS in the absence of endogenous PCNA ubiquitylation (Parker et al. 2007; Ramasubramanyan et al. 2010; Zhao and Ulrich 2010).
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