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The simplest approach is to convert the non-positive rates to a number below the minimum observable growth of 1 mm in 5 years (Condit et al. 1993a, 2006).
In the presence of another set of phenolic compounds in which the cells experienced increased lag phase and reduced biomass with increasing concentration of the phenolic compounds, the concentration at which the cells last showed observable growth had an elongated the lag phase of about 5 times that of the control, the cells stopped growing in higher concentrations.
A total of 12 independent transgenic lines were produced but none of them exhibited any observable growth alteration.
Minor but observable growth delays and increased survival was demonstrated (Additional file 1: Figure S5).
The QS mutant had no observable growth defects (see Supplementary Text and Supplementary Figure 2B, Supporting Information), except in QS-selective media.
However, concurrent decrease in kinase activity coupled with substrate overexpression causes loss of regulation of essential substrate(s) (Substrate 1) leading to observable growth defects.
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In turn, the absence of observable P2 growth at vertex for Group 1 could reflect growth over temporal-occipital regions that was insufficient to be detected at vertex.
However, for susceptibility of either plant or animal to be recognized by an observer, there would be the need for its translation into an observable movement (growth) and/or physiological change.
Similar experiments with the SsrA conditional mutant did not result in observable bacterial growth arrest.
The lag phase was similar in all cultures with little observable Af growth between 0 and 12 h (Fig 1A).
These results translated into observable cell growth phenotypes in T. brucei and L. major that were consistent with inhibition of the anticipated targets.
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