There are obligatory steps to be carried out when using the computer; every surgeon must perform the same steps, and every step is recorded, so we can check what has been performed with precision.
The FGF-induced formation of tarsal muscles involves maintenance of Twist expression in tarsal adepithelial cells followed by differentiation of Lbe/Duf-positive myoblasts, thus indicating that activation of Lbe and Duf are obligatory steps in leg muscle development.
p65 nuclear translocation and I κB α degradation are obligatory steps in the NF- κB activation [ 29].
However, our studies indicate that testing analysed strains for the presence of endobionts and assessment of the robustness of tree topologies by bootstrap analysis seem to be obligatory steps in such analyses.
Opening of mitoKATP channels and activation of PKC and p38MAPK are obligatory steps in the signal transduction cascade of IP and PP of the human myocardium with PKC activation being downstream of the opening of mitoKATP channels and upstream of p38MAPK activation.
The testing of analysed strains for the presence of mycoplasmas and other intracellular parasites in order to avoid the contamination with foreign DNA as well as assessing the reliability of the tree by bootstrap analysis seem to be the obligatory steps in such analyses.
