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An Olympus LMPlanFLN 20x/0.40 objective was used to collect the exit wave leaving the specimen.
A 40x/1.2 water objective was used together with a pinhole in the detection plain.
Olympus 10× UPlanFL N 0.3 NA air objective was used for sample alignment and large field of view fluorescence imaging.
An Olympus 4 × with a 0.13 numerical aperture objective was used to identify characteristic blood vessel patterns and to reliably relocate previously imaged areas of the cortical neuropil.
An Olympus 40 ×, 0.80 numerical aperture water immersion objective was used to acquire the images (typically 100 × 100 μm field of view, 512 × 512 pixels).
For the 2P measurement a 10× objective was used to allow for a greater working distance required by using a cuvette.
A Zeiss LSM 880 confocal microscope equipped with a Plan Apochromat 1.4 63x/1.40 Oil objective was used to visualize the hydrogels.
A Zeiss alpha Plan-Fluar 100 × 1.45 oil immersion objective was used and 24 laser shots in 0.5 µm-steps were administered over a 12 µm linear cut.
A Leica SP5 X MP confocal microscope equipped with an HCS PL APO CS 20.0 × 0.70 oil objective was used to detect reconstituted YFP.
When the binder sizes were beyond the field of vision, a ×5 objective was used.
The same microscope objective was used to collect the QD emission.
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