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Assessment of the primary study objective was performed on the DLT population.
A scan of the entire device at the 10× objective was performed using an automated stage on an epifluorescence microscope (Nikon Eclipse TE200, Melville, NY).
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An assessment for the current design and control approach in minimizing the optimization objective is performed.
From this, a simultaneous optimization objective is performed where, first, two linear transformations are learned to map the source and target data into a common subspace.
Automated image acquisition using a 2× objective is performed and the area of collagen I and fibronectin per cell is ascertained using the Metamorph® Imaging System software.
A comparison between FBA results generated through this approach and those generated from applying a more traditional biomass objective is performed.
A typical example of MABC that used markers for all three objectives was performed by Chen et al. (2000).
First, local planning with individual objectives is performed.
After 9 months, an objective evaluation was performed.
Single objective optimization was performed by the Taguchi-based signal to noise (S/N) ratio approach.
In the first step, independent optimization of objective functions was performed using Lingo 14.0 software.
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