To visualize the deposition of new plaques, we used an Olympus 20× water immersion objective to acquire image stacks of 634 × 634 × 150 μm (xyz dimensions) with 5 μm z-increments and 512 × 512 pixel resolution.
The percentage of cells with aSyn inclusions was determined by fluorescence microscopy using a Zeiss Axiovert 200 M (Carl Zeiss, Jena, Germany) wide-field fluorescence microscope equipped with a cooled CCD camera (Roper Scientific Coolsnap HQ, Martinsried, Germany) and a 63 × objective to acquire images containing at least 500 cells per strain, which were then counted using ImageJ.
Our objective is to acquire both modern and vintage timepieces for long-term value appreciation, and also take advantage of short-term strategic positions," in the international watch market, he said.
Sometimes the objective is to acquire eligibility for assuming the "sick" role and thereby being relieved of various productivity-related expectations and responsibilities.
The first objective was to acquire radiometrically normalized, multi-footprint (11, 22, 44 and 59 cm) waveform (WF) data that characterize 1064-nm backscattereflectancece on the interval scale.
In an autonomous metabolomic experiment, the objective is to acquire MS/MS data for all monoisotopic ions of biological origin.
While traditional confocal microscopes require physical translation of the sample or objective lens to acquire data in depth, the CCM captures confocal reflectance data over the 157 µm depth range of the chromatic shift in a single shot.
The objective was to acquire a general idea about the way medical students perceived organ donation and their knowledge regarding it and whether clinical exposure, which gradually increases in the five years of medical school, has any impact on it.
The objective used to acquire the original images, that were subsequently used for the analysis was a Leica HCX PL APO CS 20×/0.70 IMM UV (communication with original authors).
Although IC50 values obtained from mammalian cells expressing the hERG channel are more reliable than those from cells with heterogeneous expression of the hERG channel (10, 11), we collected electrophysiological data from scientific papers in an objective manner to acquire chemical compound information with as much structural diversity as possible.
One of his objectives was to acquire provisions for his forces and those of John Butler, who was planning operations in the Susquehanna River valley.
