The objective is split into three different tasks.
With such an objective, we split our work into two components: the structural design followed by formal modelling of the designed system for behavioural analysis.
The PL emission of the nanowire, collected by the same objective, was split by a birefringence prism into two orthogonally polarized rays and imaged on the entrance slit of a spectrometer.
On 16 December 1944, a large German offensive was launched in the Ardennes, with the German objective to split British and American forces apart and capture the port of Antwerp, an important logistical base for the Allies.
(3) The fluorescence signal collected via the objective was split into two spectral bands by a wide-field dual-imaging system (Optosplit II, Cairn Research Limited, Faversham, UK).
Emission was collected by the same objective and split by a 650 long pass dichroic mirror (z650rdc, Chroma Technology GmbH) into a red emission channel for Alexa Fluor 647 detection.
Fluorescence signals were collected through the objective and split to Cy3 and Cy5 channels through a Dual-View imager (Optical Insights, LLC), with a dichroic (Chroma, 630dcxr) to split Cy3 and Cy5 signals and bandpass filters for Cy3 (Chroma, D585/30 m) and Cy5 (Chroma, D680/30 m).
Emission was collected by the same objective and split by a polychroic mirror (z445/514/633 RPC, Chroma) into a blue emission channel for mTFP1 detection (HQ485/40m, Chroma) and a yellow emission channel for mVenus detection (Razor Edge Long Pass 514 filter, Semrock, Rochester, NY).
Fluorescence emission was collected with a 60× 1.2 NA water immersion objective and split by a 645dcrx dichroic mirror (Chroma) into short and long wavelength paths, filtered for TAMRA and Cy5 emissions using HQ 585/70 and HQ 700/75 bandpass filters (Chroma), and relayed as side-by-side images onto a charge-coupled device camera (Cascade 512B, Roper Scientific).
The beam was directed into a microscope (Eclipse T i, Nikon), with a high reflecting mirror for 800 nm as shown, to direct the beam to the objective and split the laser beam from the image for viewing on the CCD camera (DFK41BU02, The Imaging Source).
First, the dynamic model of the objective power-split HEV is built and the mode shifting process is analyzed based on the developed model to determine the reason for the system shock generated.
