Exact(2)
Decision makers expected more transparent objective settings to both OH services and the outcome of services.
With the given objective settings, the theoretical resolution was of 250 nm in x, y and 700 nm in the z plane.
Similar(58)
A pre-piloted Excel spreadsheet was used to collect information about the study objectives, settings, sample size and selection and other details of the research methods.
We collected data on patient demographics, admission diagnosis and severity of illness; study objective, setting, and design; ventilator settings; classification of pleural effusion (exudative vs. transudative); technique of drainage, including the use of imaging guidance, the level of training of the operator, and the type of drainage procedure performed; and outcomes.
"Instant Book reduces the potential for bias because hosts automatically accept guests who meet these objective custom settings they have put in place," the report said.
Prepare the z -stacks by 20× or 60× oil objectives (the settings are described in supplemental table 2).
These data will be particularly useful to develop and improve the program under objectives 1 and 2, and to assess pitfalls and strategies for implementation of the program in general settings (objective 5).
They paint an image of the camera's capabilities in the setting, but also truly tell me whether or not I like using the camera (subjective) and if it delivers crisp images under specific device and environmental settings (objective).
In cases images were taken using 20× objective, the filter settings were as below: DAPI channel - 100, TRITC channel –2200.
Images were acquired with an LSM 780 confocal microscope (Carl Zeiss Microimaging) mounted on Zeiss Axiovert 100 M equipped with Plan-Apochromat ×40, 1.3 numerical aperture (NA) oil-immersion objective, using standard settings.
Super-resolution microscopy was performed on a Leica TCS STED microscope with a 100X, 1.4 NA STED-grade objective and standard settings for emission and depletion, using atto-647N-labeled goat anti-rabbit secondary antibody (Sigma Aldrich 40839) to visualize titin.
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