Exact(2)
The diameter of the objective pupils imaged on CCD1 (12 mm for the 20×/0.5 objective and 7.62 mm for the 63×/0.9 objective) are smaller than the sensitive surface of CCD1 (12.28 mm × 12.28 mm).
Before entering the interferometer, the beam is expanded to 11 mm (1/ e width) by the beam expander, so that the objective pupils are overfilled by a factor of 1.22 for the 20×/0.5 objective and 1.92 for the 63×/0.9 objective.
Similar(58)
(typically powers would not exceed 10 mW at the objective pupil).
These were also employed to conjugate the objective pupil with the DM (L2-L4) and the with the GMs (L1-L3).
We located the SH WFS at the microscope objective pupil plane to measure the WF of the excitation beam.
A large beam diameter at the objective pupil was needed to ensure the best performance of the objective (the smallest spot on the sample).
It is in this plane that the objective pupil is filled and results in a tight focus in the focal plane.
Once Coupling Aberrations are corrected, the aberrations generated from the objective pupil to the output port of the microscope (i.e. including the conjugation lens) were measured and taken into account as a reference for all the subsequent WF measurements.
Taking into account this, the first correction was applied to the excitation beam to eliminate the aberrations introduced by the optical elements in the path that goes from the laser to the objective pupil (which we called "Coupling Aberrations").
The lateral extension of the dCV (ddCV) (estimated from the typical size of the speckle in the objective pupil (dspeckle) by ddCV ≈1.22 λfobj/dspeckle [ 27, 28], where fobj is the focal length of the objective) increases monotonically and seems to saturate at large depths (Fig. 5(c), FOV 1000 µm and Fig. 5(d), FOV 530 µm).
The instructions of the curricula included the objective that pupils be made to "understand the concept of adaptation" and the "ways" by which plants and animals adapt to the environment, as shown in the relevant excerpts (in italics and quotation marks) from the curricula and the textbooks of both pupils and teachers (Table 1).
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