The more the purification steps were used, the less the objective proteins were gained.
The objective proteins p-ERK1/2 (42, 42 kD), ERK1/2 (42, 42 kD) and β-action (42KD) were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (5% concentrated gel on 80 V and 10% separating gel on 120 V successively) and transferred onto the polyvinylidene fluoride (PVDF) membranes (90 min with 360 mA).
To achieve these objectives, protein and mRNA expression of markers of differentiation such as GFAP and NCAM as well as cytoprotection like HSP70 and mortalin were studied.
The objective of protein structure prediction is to predict the native structure of a protein from its sequence.
Objectives: Normalized protein nitrogen appearance (nPNA), also known as protein catabolic rate (nPCR), reflects the daily protein intake in maintenance hemodialysis (MHD) patients.
Understanding the relationship between primary and tertiary structure in proteins is one of the main objectives of protein sequence analysis.
Concepts presented here may be useful for objective clustering of protein functions and deriving a comprehensive protein interaction map.
Objective: Parathyroid hormone related protein has roles in normal fetal growth, placental calcium transport, and vascular tone regulation; these factors are compromised in growth-restricted fetuses.
An important objective of computational protein design is the generation of high affinity peptide inhibitors of protein peptide interactions, both as a precursor to the development of therapeutics aimed at disrupting disease causing complexes, and as a tool to aid investigators in understanding the role of specific complexes in the cell.
Here we present a qualitatively distinct and novel approach, called Comparative, Objective Measurement of Protein Architectures by Scoring Shifts (COMPASS), which identifies the best structures from a set of structural models by numerical comparison with a single, unassigned 2D 13C-13C NMR spectrum containing backbone and side-chain aliphatic signals.
