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Specimens were analysed on an Axio Imager.Z1 (Zeiss) equipped with an AxioCam MRc3, × 0.63 Camera Adaptor and the ZEN 2012 blue edition software using × 10 objective (Plan Apochromat × 10/0.45 M27) or × 20 objective (Plan-Apochromat × 20/0.8) and evaluated with Image J software.
Cells were imaged using a Nikon Eclipse Ti microscope (Nikon) equipped with a Neo-Zyla sCMOS camera (Andor), LED illumination (CoolLED) and a ×60 objective (Plan Apo 60 × /1.4 Oil, Nikon).
Microscopy data for gliding velocity evaluation and part of the data for sliding velocity evaluation were acquired using an Axio Observer Z3 microscope (Carl Zeiss AG, Germany) equipped with a 63×, 1.46 NA, WD = 0.1 mm objective (plan, apochromat, Zeiss) in combination with an EMCCD iXon-3 camera (Andor, Northern Ireland) and MetaMorph software (Molecular Devices, USA).
The excitation light was focused on the sample with an oil immersion objective (Plan Apochromat, ×60, NA 1.42, Olympus).
The beam was reflected with a dichroic beam splitter (Chroma 505DCXR, Rockingham, VT, USA) to the microscope objective (Plan Apo, ×100, oil immersion, Nikon).
The excitation beams were focused on the sample with an oil immersion objective (Plan Apochromat, ×60, NA 1.42, Olympus Corporation, Tokyo, Japan).
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A 100x TIRF objective (Plan-APOCHROMAT 100 × /1.49 Oil, Nikon) with additional ×1.5 magnification was used to collect fluorescence onto an EMCCD camera (iXon Ultra 897, Andor), yielding a pixel size of 107 nm.
A main objective planned for The King of Fighters '95 was to properly introduce Iori as Kyo's rival.
A Zeiss 63x oil-immersion objective (Plan-Apochromat, NA 1.4) was used.
Epifluorescence images were obtained with an epifluorescence microscope (Zeiss) using a 100× objective (plan-apo) coupled to a "piezzo" enabling acquisition of images every 200 nm in the Z plane.
Fixed cells were analyzed with a laser-scanning microscope (LSM 510 META, Carl Zeiss), with a laser line at 458 nm, and an objective Plan-Apochromat 40× Oil Ph3.
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