Immunofluorescence images were obtained with a Nikon PM 800 confocal microscope equipped with a digital camera controlled by Spot software (Diagnostic Instruments Inc.) and using a × 100 objective (numerical aperture=1.4) or with a Zeiss LSM 510 confocal microscope using a × 63 objective (numerical aperture=1.2).
But the Supreme Court suggested in 1986 that there may be a 50percentthresholdld, and on Monday it made that suggestion into what Justice Kennedy called "an objective, numerical test" that "draws clean lines for courts and legislatures alike.
For many of our parents, success is simply not measured by their child's progress, contentment or kindness; it can only be understood in objective, numerical terms, pitched against the attainment of other children.
The second method, termed the "transformational approach," would deploy a "metric-based performance measurement system" with objective numerical thresholds or benchmarks corresponding to the four statutory rating categories: outstanding, satisfactory, needs to improve, and substantial noncompliance.
In order to achieve the objective, numerical modeling was carried out using the finite-element model HYDRUS-2D.
In addition, neural network (NN) models were created using three objective numerical inputs (loudness, sharpness, and roughness) of the SQ metrics and one subjective output ("pleasant").
This problem is exacerbated by the nature of my discipline, in which grading is less likely to involve objective numerical or multiple-choice assessment and more likely to entail subjective assessment of the strength of reasoning conveyed by students' narrative answers.
Setting objective numerical goals ensures that minimal bias influences their execution, and eventually guarantees that failing programs have the opportunity to be corrected.
Samples were excited with an Ar-Kr laser source (514.5 nm), providing a photon flux lower than 60 W/cm2 by a × 50 microscope objective (numerical aperture 0.75, light spot diameter <2 μm).
A Leica SP2 Confocal Laser Scanning Microscope (Leica Microsystems GmbH, Wetzlar, Germany) equipped with a 63× water immersion objective (Numerical Aperture = 1.2) was used for imaging with either 1 or 2 x electronic magnification.
A confocal Zeiss Pascal 5 microscope and a Zeiss 10X objective (numerical aperture = 0.3) were used too obtain images for quantification; a Zeiss 40X objective (numerical aperture = 1.3) was used for the rest.
