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We acquired time-lapse micrographs of isotropic spreading cells with both bright field illumination (BF, Fig. 1A) and total internal reflection fluorescence (TIRF, Fig. 1B) using a 20× objective (Movie S1).
Thus, mice were anaesthetized and their spleens exposed and visualized under a high-speed multiphoton confocal microscope fitted with an inverted 63x glycerol objective (Movie S1).
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"The objective with the movie is to create an experience that's more exciting than playing with the toys, which shouldn't be hard to do," says Mark Gill, a producer and former president of Miramax, which, once upon a time, distributed the product-pushing Pokemon: 4Ever.
"I'm fascinated by our subjective perception of reality, that we are all stuck in a very singular point of view, a singular perspective on what we all agree to be an objective reality, and movies are one of the ways in which we try to see things from the same point of view".
And as such it's almost impossible to stay objective when watching a movie, or play.
I am objective-correlative-ing that movie scene to the max, but basically whenever I feel like I am supposed to have a specific, moral, prescriptive opinion on something like this I say that line to myself, and probably have a sad little lie-down or similar.
When you're composing music for a movie, your main objective is to guide the viewer emotionally through the movie.
But infighting between hardware and movies hindered that objective from the start, as did misaligned incentives that led Sony to wrestle with how to build devices that let consumers download and copy content without undermining sales at its music labels or film studios.
The objective was to record 3D movies via a main surgeon's perspective, to watch files using virtual reality headsets and to validate pedagogic interest.
The principles of "Moneyball" — celebrated in a popular book and movie — spread rapidly, with objective analysis complementing traditional scouting to form a better understanding of talent and value.
This research focused on studying user's affective (subjective) and physiological (objective) responses to the 3D emotional movie.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com