Each tissue section was photographed and analyzed using a microscope (20× objective) coupled to a computerized morphometry system (Leica Qwin V3, Leica).
Slices were viewed with infrared differential interference contrast imaging through a 40× water-immersion objective coupled with 2× premagnification (Optovart; Zeiss, Oberkochen, Germany) and a CCD camera (C79; Hammatsu, Tokyo, Japan).
Microscopic images were also taken for comparison on a commercial inverted microscope (BX-51, Olympus, Center Valley, PA) equipped with a 20× objective coupled to a commercial CCD camera (DP-71, Olympus, Center Valley, PA) Sample holding was handled in two ways.
Specifically, for microscopy of specimens on slides, a Leica DC100 microscope was used with a × 20 objective coupled to a 1-MPixel Leica DC100 video camera wired to a 2-GHz PC running Leica IM1000 software (Leica Microsystems GmbH, Wetzlar, Germany).
Imaging was performed at 37°C in 5% CO2 using an Olympus IX81 inverted microscope with a 60x 1.42NA oil immersion objective coupled to an Ultraview Vox spinning disk confocal system (Perkin Elmer) fitted with a C9100-13 EM-CCD camera (Hamamatsu Photonics Limited, UK).
Images of the cell membrane inside the patch pipette were captured at a rate of ~7.5 frames per second (125 ms exposure) at a resolution of 61.5 pixels/μm using a Plan Apo (100x) DIC oil objective coupled with a Coolsnap ES camera and 4x relay lens (Nikon Instruments Inc, Melville, NY).
The demands of the many measurements needed to meet the program objectives, coupled with the constraints associated with spacecraft safety and the orbital geometry, led to the adoption of an automated science-planning tool to optimize science data collection.
Fraction 18 from the Poros HQ column was digested with trypsin and peptides were analysed by liquid chromatography-MS/MS using 75-µm×15-cm 75-µm×15-cm 75-µm×15-cm C18bjectives) coupicofritan LTQ mass specolumnser aNewpeptides were eluted using a 10–85% MeObjectivest in 0.5% acoupledcid.
Interferometric reflectance spectra of PSiO2 samples are collected using an Ocean Optics CCD USB 4000 spectrometer (Dunedin, FL, USA) fitted with a microscope objective lens coupled to a bifurcated fiber optic cable.
The authors consider that such an objective, especially coupled in with FEA still can lead to high localized stresses which therefore do not improve the strength of the structure.
