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Wilson nonetheless carried his main objective back to America, a treaty for a "League of Nations," intended to prevent future wars.
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Backscattered laser light from the sample was also collected by the focusing objective, reflected back to the beam splitter, and combined with the return beam from the reference arm.
An excitation filter (D460/50x, Chroma) was placed between the spot and condenser lenses, and an emission interference filter (Chroma D550/50 m) was placed as close as practical to the objective back focal plane.
This gives a number between 0 and 1, indicating how effective is the transformations in taking an initial state to the objective state and back to the initial state in twice of time (the reset phase).
Raman scattering was collected through the same objective and transmitted back to the spectrograph and EMCCD (Newton 970, Andor).
An axicon lens creates a Bessel intensity distribution that is relayed on the beam steering device and further in the objective back-focal plane to produce a Bessel beam illumination on the sample.
Laser powers were measured at the objective back aperture and corrected for objective transmission efficiency to represent the power at the sample.
Alternatively, polarized emission from the dipole converts to a spatial representation at the objective back focal plane [7], [8] that the tube lens images as the PSF at the camera.
Illumination was 7 mW at the objective back aperture, at 810 nm for the FRET probes and at 910 nm for EGFP and the GCaMP2 probes.
The power at the objective back aperture during ablation was 90 100 mW.
The laser beams were attenuated using a half wavelength plate and expanded using a custom telescope that could be configured for both confocal illumination (collimated beam output) or a converging beam at the objective back focal plane.
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