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Adipogenic lineage commitment was evaluated in adipogenic induced cultures, by staining for 5 min with an Oil red O working solution (3 parts of 0.5 % Oil Red O in isopropanol diluted with 2 parts of distilled water).
The Oil Red O working solution was prepared as described [25, 27].
The 3T3-L1 cells were stained with Oil Red O working solution for 30 min at room temperature.
A fresh 60% Oil Red O working solution was prepared from a stock solution (0.7 g Oil Red O in 200 mL isopropanol) and filtered through a 45 µM syringe filter.
Lipid accumulation in adipocytes was visualized by staining with Oil Red O. Briefly, differentiated adipocytes were washed with phosphate buffered saline (PBS) and fixed with 10% formaldehyde in PBS for 4 min. After two washes with water, cells were stained for 1 2 hrs with Oil Red O working solution (0.3% (wt/vol) in 60% isopropanol).
Then, cells were stained with the Oil Red O working solution for 10 min.
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LONG BRANCH "O," works based on that which is round, curvy or looped.
Specifically they link E to sensitivity to rewards, N to sensitivity to punishment, A to altruism, C to impulse control, and O to working memory and attention (see also DeYoung, 2010).
The Oil Red-O working solution was prepared by mixing 15 ml of a stock (0.5% in isopropanol) and 10 ml of distilled water and filtering through a PDVF membrane (0.2 µm) filter.
The organization Obed works for approached the government for help with this project in 2008, after receiving regular calls from individuals hoping to track down their family members.
Oil Red O (Sigma Aldrich) working solution was prepared by adding 20 ml dH2O to 30 ml 1% Oil Red O in isopropanol.
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