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LONG BRANCH "O," works based on that which is round, curvy or looped.
The Oil Red O working solution was prepared as described [25, 27].
Number of workers of skill category o working in period s.
The 3T3-L1 cells were stained with Oil Red O working solution for 30 min at room temperature.
A fresh 60% Oil Red O working solution was prepared from a stock solution (0.7 g Oil Red O in 200 mL isopropanol) and filtered through a 45 µM syringe filter.
Lipid accumulation in adipocytes was visualized by staining with Oil Red O. Briefly, differentiated adipocytes were washed with phosphate buffered saline (PBS) and fixed with 10% formaldehyde in PBS for 4 min. After two washes with water, cells were stained for 1 2 hrs with Oil Red O working solution (0.3% (wt/vol) in 60% isopropanol).
Then, cells were stained with the Oil Red O working solution for 10 min.
For lipid droplet staining, the cells were fixed with 10% formalin and stained with Oil Red O working solution.
After 5 min, the isopropanol was discarded and the cells were incubated with Oil Red O working solution (Fisher Scientific) for 5 min at room temperature.
After 2 weeks, the cells were fixed in a 4% PFA for 20 minutes and incubated for 10 minutes in an Oil Red O working solution.
After that the cells were washed with 60% Isopropanol, and added 3 ml of Oil Red O working solution to the fix cells.
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