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A mode locked Ti:sapphire laser (Coherent Chameleon, USA), running at 80 MHz, and tunable from 710 950 nm was attached to an upright Zeiss Axioplan-2 microscope with a Zeiss 510 META/NLO scan head and was used to acquire spectral images with two-photon excitation [40] for sRET analysis [16], [17].
Terminate "COMODO Scan".
ESA can be described as I_{P - f }propto sqrt {Dnu } C_{0} A (1)[31, 32] 32], and the Faradaic peak current I p−f depends on the diffusion coefficient (D), initial concentration (C o), scan rate (v), and electroactive surface area (A).
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Two women (4 %) were receiving exogenous hormone replacement therapy before their pre-RRSO and post-RRSO scans.
All subjects underwent one FDG scan (to measure CMRGlu [ 14]) and two sets of three O scans (CO, H2O, and O2) to measure CBV, CBF, and CMRO2 [ 11– 13].
The P values derived from the t test comparing the BPE-related measures in the pre-RRSO scans versus the post-RRSO scans for a range of values of the R%cutoff are shown in Manhattan plots in Fig. 2.
Toward this end, we also used leave-one-out cross-validation (LOOCV) to compare AUCs computed based on relative changes of BPE between post-RRSO scans and pre-RRSO scans, derived across the same range of the R%cutoff values (from 0%to100%00 %; see Additional file 1).
A CMRO2 parametric image was derived from the O scans using a previously described method [ 15, 16], and a CMRGlu image was derived from the FDG scan for each participant [ 14– 16].
Of the 55 women, 5 (including 3 women who developed breast cancer after RRSO) were excluded from our analysis because their DCE pre-contrast series in either the pre-RRSO or post-RRSO scanning were incomplete, so we were unable to quantitatively compute BPE.
In addition, no previous study researchers have evaluated the changes in BPE and FGT measures between pre-RRSO and post-RRSO scans for predicting the development of breast cancer post-RRSO, which is an important clinical endpoint of interest.
Sagittal sections were made at 300-μm intervals and stained with Safranin-O to scan the amount and distribution of the glycosaminoglycans.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com