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In the High P(O) condition, the light came on in 40 out of 50 trials, that is, P O) =.80, whereas in the Low P(O) condition, it came on in 10 out of 50 trials, that is, P O) =.20.
To check the oxygen deficiency, we calculated the EELS signal of O using the EELS signals of Ti and Sm, assuming a stoichiometric O condition, i.e., O/Ti = 3 and O/Sm = 1.5 for SrTiO3 and Sm2O3, respectively.
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In order to know whether P was actively secreted by the OCC, we measured the P concentration in the OCC, CU and O conditioned media.
In the OCC and CU conditioned media the P concentration was 53±17 nM and 16±12 nM, respectively; but in the O conditioned medium no P was detected (Fig. 2C).
Moreover, P was found in the OCC and CU but not in the O conditioned medium, indicating that only the cumulus cells secrete P to the medium, in agreement with the observations in other species [5] [8].
In order to obtain the CU and O conditioned media, the isolated OCC were passed several times through a fine-bore glass pipette with 0,1 mg/ml of hyaluronidase.
Further, the pHO response of these mutants in both high and low [K+]O conditions was very similar to that of wild-type K2P2.1 (TREK-1) under high [K+]O conditions.
After 24 h, N, A, H or O conditioned media from HBMEC3, SHSY-5Y or HFA were collected and filtered through a 0.22 μm filter and stored at −80 °C until use.
Under O-rich condition, the chemical potential of O can be calculated from the ground state energy of O2 molecule, while the chemical potential of Ti is fixed by Equation (3).
The formation energy of the (NH4 Zn complex could be lower than that of VZn at O-rich condition, making its formation at zinc site energetically favorable.
On the other hand, for the p-type doping, Li-doped ZnO nanosheet has acceptor level at 0.68 eV above valence band maximum (VBM) and is more energetic favorable than the Li-Ni doped ZnO in O-poor condition.
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