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LNs were disrupted against a nylon screen and treated with Collagenase D (Roche) for 30 min at 37 °C.
The mixture was stirred gently for 4 min, and washed mince was filtered with a layer of nylon screen.
Finally, the bottoms of the columns were closed with a nylon screen of 77-μm mesh to prevent soil from escaping during the percolation of the solution and another nylon screen (50% shading) was used to maintain the soil in the column.
To measure the swelling ratio of microspheres, a tea bag (i.e., a 100 mesh nylon screen) containing pre-weighed dry samples was immersed entirely in the brine (with the composition given in Table 1).
To measure the ESR, tea bags (i.e., a 100 mesh nylon screen) containing 2 g of the prepared particle gel were immersed entirely in the solution of the acidic or basic water and CaCl2 (according to the experimental design composition presented in Table 2) and allowed to soak for 2, 15, 30, 40 and 50 days to make sure of the equilibrium state.
The dispersed testicular cells were sieved through a nylon screen (mesh size 50 µm) to eliminate the non-dissociated cell clumps.
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In the sun fresh meat could take a full day to dry out; today the sliced, spiced, brined meat rolls on nylon screens through a drying oven with fans and exhaust pipes to draw out moisture, reducing the drying time to a few hours.
Organs were mashed onto nylon screens (Sefar Italia, Torino, Italy) and washed in phosphate buffered saline (PBS) with 0.5% of bovine serum albumin (BSA, Sigma-Aldrich) and 2 mM EDTA (Mallinckrodt Baker, Philipsburg, NJ, USA).
To facilitate changing solutions, put the leaf in a shallow glass container and cover it with nylon screening.
Place plastic or stainless steel weights over the nylon screening to keep the leaf flat and to prevent flotation; be careful not to put the weights directly over the leaf (Appendix S1A ). 2. Immerse the leaf in a 5% solution of NaOH (Appendix S1B ) at 40 54°C (Appendix S1C ) for one to several days (the time needed will vary from leaf to leaf).
The dispersed CNT were then filtered from the solution using a 20-μm nylon mesh screen followed by a 0.2-μm polytetrafluoroethylene filter.
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