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Proteins were separated on a 10% SDS-PAGE gel and blotted onto a nylon membrane.
Nylon membrane was used as the array support.
Digested DNA was electrophoresed through 0.9% agarose gels, transferred to a nylon membrane (Roche), and UV cross-linked.
Sonicated samples were filtered through a 5 µm nylon membrane to remove possible minerals and coarse materials.
The DNA digestion solution was diluted 3-fold and filtered through a 0.2-µm nylon membrane (Agilent).
Before use, this solution was filtered onto syringe filter with nylon membrane (pore diameter 0.22 μm).
DNA was transferred to Hybond N+ nylon membrane under alkaline condition.
The solution was passed through a 0.22-μm nylon membrane filter.
All samples were filtered through a 0.2-μm nylon membrane before injection into the HPLC instrument.
The nanofibers were then filtered using nylon membrane filter paper of 0.45-μm pore size.
4) The pattern, invisible at this point, is transferred to a nylon membrane.
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