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Blood was collected for lymphocyte subset analysis, quantitation of plasma HIV-1 RNA, and nutritional assays.
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So they could do a full nutritional assay.
From a total of 759 nutritional phenotype assays, 160 were positive (indicated by a 40%% growth enhancement relative to the control) for C. sordellii strain 8483, while 132 were positive for C. difficile strain 630 (Additional file 1: Table S1 and Additional file 2: Table S2).
We assayed nutritional indices in pools of 10 adult males from each line aged 3 6 days after eclosion.
The significance of gene expression differences between the nutritional conditions was assayed by analysis of variance followed by an analysis of the two contrasts "Fst16-Fed" and "Fst48-Fed".
We assayed nutritional indices in the DGRP (Mackay et al. 2012), a collection of approximately 200 inbred lines of Drosophila melanogaster derived from wild-caught females (2003, Raleigh, NC).
The most significant improvement that accounts largely for the success of the two-hybrid system has been the conversion from a color-based assay to a nutritional selection via inclusion of the HIS3 gene as a transcription reporter in addition to LacZ.
These findings are reminiscent of those for C. elegans long lived mutants assayed either under nutritional stress [ 22] or benign conditions [ 24] and are consistent with the idea that the tradeoffs predicted by life history theory [ 25] may only manifest themselves in nematodes experiencing some form of stress - either exogenous or endogenous stress (e.g., mitochondrial dysfunction).
Nevertheless, these assays are widely used in (nutritional) studies focusing on the effects of flavonoids.
The nutritional PM analysis consisted of 190 assays of carbon source metabolism, 94 assays of phosphorous and sulfur source metabolism, 95 assays of biosynthetic pathways, and 380 assays of nitrogen source metabolism [ 35].
The nutritional components in bee pollen were also assayed in order to promote its consumption.
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