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We used only rich nutrition medium to exclude nutritional stress.
For studying the effect of the antimicrobial agents on the ultra-structure of microbial cells, tested microorganism was cultured on the appropriate liquid nutrition medium to get a cell suspension of 5x106 cells/mL, and then mixed with the selected polymer of the previously recorded MIC.
For studying the effect of the antimicrobial agents on the physiological parameters of microbial cells, tested microorganism was cultured on the appropriate liquid nutrition medium to get a cell suspension of 5x106 cells/mL, and then mixed with the tested polymer of the previously recorded MIC.
At this point, the adipocyte nutrition medium was withdrawn and the cells were washed 5 times with the differentiation media.
We have to point out that the light intensity that the chlamydomonas strains could support depends strongly on the growth illumination and the physical phase of the nutrition medium.
Three days after the initiation of the cells differentiation, the differentiation media were replaced with adipocyte nutrition medium containing 3% FCS, d-biotin 8 ug/ml, 0.5 ug/ml bovine insulin and 400 ng/ml dexamethasone for a period of 16 days (terminal phase of differentiation).
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The growth of UTEX 2341 reached the exponential phase on the 1st day in low-nutrition medium.
Axenic cultures were grown in full-nutrition medium [ 115] for 7 d after imbibing sterilized seeds for 3 d at 4°C.
40 embryos collected from GF females were transferred to a fresh low-nutrition medium poured in small petri dishes (ø = 5cm).
We evaluated the FA content of this strain, compared to WT, using gas chromatography (GC), under different conditions of nutrition [rich medium (YPG) and poor medium (YNB, low or high C/N)].
To evaluate evidence regarding grocery store tours as an effective nutrition education medium for improving nutrition knowledge and food-related behaviors.
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