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Six nutrients: yeast extract, (NH4 2SO4, K2HPO4, NaCl, MgSO4·7H2O and CaCl2 were detected within pullulan production in flasks.
Since the assay utilizes a mutant strain of yeast that cannot grow on media without these nutrients, yeast will only grow when the binding domain and activating domain (and thus, the proteins of interest) interact.
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A study conducted by Thaniyavarn et al. (2003), then indicated that Bacillus licheniformis grown in nutrient yeast potato dextrose medium produced five surfactin homologues as detected by LC MS analysis.
The results showed that the antagonistic activity of C. laurentii obtained from the culture media of nutrient yeast dextrose broth (NYDB) amended with chitin at 0.5 1.0% was improved greatly compared with the case that without chitin.
Cultures of P. aeruginosa PAO1 were grown in nutrient yeast broth (NYB) to exponential or stationary phase and total RNA was extracted.
Strains were routinely grown in nutrient yeast broth (NYB; 2.5% [wt/vol] nutrient broth, 0.5% [wt/vol] yeast extract) with shaking, or on nutrient agar (4% [wt/vol] blood agar base, 0.5% [wt/vol] yeast extract).
Autolyzed Yeast Calcium Caseinate Gelatin Glutamate Glutamic Acid Hydrolyzed Protein Monopotassium Glutamate Monosodium Glutamate Sodium Caseinate Textured Protein Yeast Extract Yeast Food Yeast Nutrient.
Three nutrients including yeast extract, tryptone, and FeSO4·7H2O were screened as significant nutrients for ABE production.
The hypothesis was that the nutrient extract was able to provide all growth nutrients for yeast, being the sole carbon source and sole the nitrogen source.
Higher protein content (per gram of cell mass) was observed in complex media containing rich nutrients like yeast extracts than in synthetic media.
Statistical experimental designs were used to select essential nutrients for yeast fermentation, and its optimal concentrations were estimated by Response Surface Methodology.
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