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Despite numerous transgenic lines are available, histone modifications on epigenetically inactivated 35S promoters have not been studied yet in tobacco or related Petunia species (both Solanaceae).
In combination with the availability of numerous transgenic lines expressing tissue-specific fluorescently-labelled reporters, this allows real-time, in vivo visualization of various processes such as cell migration and organogenesis.
We have generated numerous transgenic lines with the phat-1 reporter in wild-type animals and have not observed significant gland defects associated with the presence of this (or other) transgenes.
We obtained numerous transgenic lines from two independent transformation experiments, one with a construct containing the PR1a promoter and the other with a construct containing the El2-35S-Ω promoter.
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The increasing popularity of the Cre/loxP recombination system has led to the generation of numerous transgenic mouse lines in which Cre recombinase is expressed under the control of organ- or cell-specific promoters.
To date, numerous transgenic fish lines that express engineered versions of Gal4 in specific cells, organs, and tissues have been generated and used for various aspects of biological studies.
A major advance has been the generation of numerous transgenic mouse lines that express EGFP (enhanced green fluorescent protein) in defined cell populations [8], [9].
Numerous mouse transgenic lines have now succeeded in partially reproducing its lesions: the extracellular deposits of Aβ peptide and the intracellular accumulation of tau protein.
Experiments to address cell-type specificity are actively being pursued in the laboratory and are an important next step, but are at least a year from completion due to the generation of numerous new transgenic lines to address these questions.
In summary, with the DsDELGT4 system, we have successfully obtained numerous tissue-specific transgenic lines and created mutants affecting both known and novel genes.
In contrast to numerous analyses of independent transgenic lines, much less attention has been paid to analyses of genetically identical clones [ 16, 17], which could bring valuable information about the variability of transgene expression independently of the positional effect.
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