The protocol was designed to increase retention of rare transcripts, efficiently remove unwanted transcripts, have the capacity to analyze numerous transcripts simultaneously, and reduce the number of false positives.
The finding of numerous transcripts for serpins and cystatins in the D. variabilis midgut suggests an important role for those proteins in innate immune defense.
Water deficit activated the expression of numerous transcripts associated with glutamate and proline biosynthesis and some committed steps of the phenylpropanoid pathway that increased anthocyanin concentrations in Cabernet Sauvignon.
A. glabripennis produces a large number of transcripts predicted to break down plant cell wall carbohydrates [ 31, 37], while in contrast, the gut community expressed numerous transcripts predicted to assimilate and ferment wood sugars (i.e., glucose and xylose).
Concerning amino acid biosynthesis, the accumulation of numerous transcripts associated with glutamate and proline (PRO) biosynthesis is upregulated by water deficit.
In this real-life application, RScan is very convenient for the researchers who might produce or obtain numerous transcripts by biological experiments and would want to know if some of them have similar structures with known ncRNA and also to deduce their possible functions.
Numerous transcripts spanning enzymatic domains and LTRs of Sinbad, from at least six developmental stages of S. mansoni, have been sequenced [ 40], and of these, the ESTs most closely resembling Sinbad are composed entirely of contiguous open reading frames, suggesting non-mutated copies.
Accordingly, we looked for cis-cis interactions and identified numerous transcripts under epistatic regulation.
Many of these transcripts represented portions of the genome that were not previously recognized as being, or predicted to be, transcribed, and included numerous transcripts in antisense orientations.
