Mounting evidence implicates numerous protein interactions in aggregate formation, a phenomenon also now recognized in different diseases.
Yeast two-hybrid, which was first applied by Fields and Song [ 24], is a high-sensitivity technology that studies protein interactions; it has been used to identify numerous protein interactions.
Talin is a large multidomain molecule that makes numerous protein interactions and has at least two separable functions: modulating integrin affinity and linking integrins to actin [ 5].
As reviewed in Suresh et al. [ 11], numerous protein interactions described for the RanBPM protein suggest its multiple roles in the regulation of protein stability, cell cycle regulation and other as yet undefined cellular processes.
However, the gross phenotypic changes associated with inactivation of an entire GTPase pathway by this method makes it difficult to understand the details of the numerous protein interactions that regulate Golgi function.
Recently, proteomic technologies have created new possibilities in the discovery of novel drug targets and have been applied to study numerous protein interactions, to measure binding affinities, specificities, and levels of protein expression in various preparations, and in the investigation of various disorders (2- 6).
The MiMI tool provides access to the knowledge and data that have been merged and integrated from numerous protein-interaction databases, and it augments the information from many other biological sources.
Here, we show that the YFP BiFC overcomes these limitations and enables a detailed analysis of numerous protein-protein interactions along the bacterial Tat pathway of live cells.
The data, therefore, are strongly suggestive that the numerous protein-protein interactions that occur in the intracellular space are an additional control factor in the formation and stability of proteins.
Being a prototypic adapter protein, the modular architecture of Nck allows for numerous protein-protein interactions.
EGF_CA domains, present in a large number of membrane-bound and extracellular proteins, play a crucial role in numerous protein-protein interactions [ 80].
