This approach is capable of analyzing for the presence of numerous transcripts when polymerase chain reaction (PCR) is subsequently employed for individual gene-specific sequence amplification (HD-MRT-PCR).
Single-cell polymerase chain reaction (PCR) poses numerous technical challenge, and the most published protocols require cells to be sorted into very small volumes of liquid, often less than 1 2 μL [9 11].
While numerous microarray and large-scale quantitative reverse transcription polymerase chain reaction (RT PCR) studies overall have supported gene-dosage effects, they have also invariably reported cell line-, tissue-, age- and gene-specific departures from it.
We discovered and confirmed numerous sequences of the candidate genes by using quantitative polymerase chain reaction and in situ hybridization.
Polymerase chain reaction, (PCR), a technique used to make numerous copies of a specific segment of DNA quickly and accurately.
Despite the detrimental effect of the fixative, numerous studies using archived FFPE samples have generated satisfactory reverse-transcriptase polymerase chain reaction (RT-PCR) data [ 9- 13].
The need for rapid diagnostics at the time of admission for patients with suspected leptospirosis has led over the last two decades to the development of numerous assays to detect antigen in a range of samples using the polymerase chain reaction (PCR).
More recently, studies have utilised reverse transcription polymerase chain reactions (RT-PCRs) for a variety of mRNA transcripts of numerous genes, including mucin1, carcinoembryonic antigen (CEA), p53, KRAS, FHIT, CDKN2A, survivin and livin [24,27,28].
We found evidence of numerous SPINs in the genome of the copperhead (fig. 5) but found none in the python (corroborated by polymerase chain reaction [PCR] and Southern hybridizations; Feschotte C, unpublished data).
