Comparing numerous normal tissues and corresponding solid tumor samples, Feinberg's lab identified hyper-variable chromosomal regions named Variably Methylated Regions (VMRs).
GeneTIER depends on an extensive database that has been built using publicly available microarray and RNA sequencing datasets and is composed of several million expression values for numerous normal tissues.
Here, we have characterized the methylation profile of the nc886 region in numerous normal tissues and observed variable methylation profile in which 76% of the normal samples analyzed were predominantly methylated on one allele and, when informative, this was the maternal chromosome.
In contrast, the MUC4 apomucin is expressed in numerous normal human tissues like the stomach, ovary, salivary gland, colon, lung, trachea, uterus, and prostate (Audie et al, 1993; Reid and Harris, 1998; Buisine et al, 1999; Gipson et al, 1999).
IL-15 mRNA is widely expressed in numerous normal human tissues and cell types, including activated monocytes, mast cells, dendritic cells, and fibroblasts [ 52, 53], where it is subject to tight regulation manifest primarily at the translational level.
It plays numerous roles in normal tissues but also has been implicated in inflammatory processes, multiple drug resistance, angiogenesis, tumorigenesis, water homeostasis, and altered viscoelasticity of extracellular matrix.
The expression of TRAIL and its receptors has been widely described in numerous normal and cancerous tissues [ 8– 13].
MALDI-IMS under various forms has already been successfully used to characterize the expression of proteins and other organic biological compounds in numerous normal and diseased tissues (Chaurand et al. 2005).
However, since PI3K and Akt mediate many signalling pathways important for numerous cellular functions in normal tissues, it remains to be determined whether these agents have sufficiently wide therapeutic index to be used in the treatment of cancer.
Unlike other TNF family members, such as TNFα or CD95L/FasL, TRAIL was detected in numerous normal as well as malignant cells and tissues [ 1, 2].
With IMRT, high doses to these selected normal tissues can be avoided by applying numerous radiation fields of varying intensities from different directions.
