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Two different analysts performed all steps in the analysis and numerous negative controls were included.
During the PCR process, strict precautions to minimize the cross contamination were adopted and numerous negative controls were included to monitor their effectiveness (Snounou & Singh, 2002).
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The concordance of the data between these antibodies together with numerous positive and negative controls described in Smith et al (2008) support the notion that the staining detected in gastric cancer and other samples reflected the presence of the CD133 protein as reported.
Finally, in consideration of potential global changes of a selected set of genes, numerous positive and negative controls were included in the array design; including genes characteristic of some tumors (e.g. the estrogen receptor for a subset of breast tumors) and many 'housekeeping' transcripts (e.g. β-actin) commonly used to normalize quantitative PCR studies.
Negative controls: Neg.
Numerous other loci on the array served as negative controls.
This report prompted us to repeat our experiments numerous times, using a variety of positive and negative controls.
Harmful algal blooms (HABs) are a worldwide problem with numerous negative effects on water systems, which have prompted researchers to study applicable measures to inhibit and control them.
While most of the blame lies with George W Bush, numerous negative trends had preceded him.
Childhood maltreatment has been linked to numerous negative health outcomes.
Negative controls.
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