Some factors show numerous low-affinity sites, usually attributable to a poorly-defined input weight matrix.
Therefore a key feature of PPARs appears to be that they act as receptors that have numerous ligands that each binds to the receptors with relatively low affinity.
A single molecule would have a low affinity but the creation of individual protein subunits with numerous oligosaccharide binding sites assembled to a filamentous structure leads to increased target avidity.
In contrast to the FG motifs present on numerous Nups, which bind somewhat indiscriminately to multiple members of the β-karyopherin family with low affinity, Nup53p contains a 30-amino-acid residue segment that binds specifically and with relatively high affinity to Kap121p.
One of the functional advantages of disorder is related to an increased plasticity that enables IDRs to bind to numerous structurally distinct targets [79], [80], [81] and allows protein interactions to occur with both high specificity and low affinity [27], [30], [31], [34], [82], [83], [84], [85], [86].
We found that PC downregulated the expression of numerous genes classified in immune response, inflammatory response, and angiogenesis, including chemokine (C-C motif) ligand 5, Fc fragment of IgG, low affinity IIIb receptor (FCGR3B), and leukocyte immunoglobulin-like receptor, subfamily B member 3 (LILRB3).
Even so, the low affinity binders can undergo in vitro affinity maturation to improve their binding affinity and specificity5,39,42.
Ent2 exhibits a low affinity for guanosine and cytidine but a high affinity for inosine.
That part of the molecule facing the cytoplasm has a high affinity for Na+ and a low affinity for K+, while that part facing the outside has a high affinity for K+ and a low affinity for Na+.
Based on their affinity for monomeric IgG the receptors are divided into high affinity CD64 and medium to low affinity CD32 and CD163,4.
Among these families, NRT1/PTR belongs to the low affinity transport system, and NRT2 belongs to the high affinity transport system22.
