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Numerous isolates were also obtained from other patients with encephalitis (33, 34 ).
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Klebsiella species were the most numerous isolates which were consistently isolated across time (94); the other isolates included Citrobacter species (55), Enterobacter species (35), Pseudomonas species (19), Providencia species (10), Salmonella species (4), Non Group A streptococcus (4) Staphylococcus aureus (4), E. coli (3), Acinetobacter spp. (3), and Shigella flexneri (1) (Table 1).
A total of 25 flaA-SVR types and 11 flaA peptides were identified among the numerous isolates that were analysed, demonstrating the presence of a heterogeneous population.
As with AKSSuT isolates, CGB subtype isolates were numerous in cattle from 1997 to 1998, but the number dropped markedly in 2002 and 2003.
These isolates were subcultured numerous times from hyphal tips to ensure purity of each isolate.
While only two recombination events characteristic of SWIO islands isolates were identified, numerous re-assortments events were detected between EACMV and EACMKV, which seem to almost freely interchange their genome components.
These isolates were resistant to numerous classes of antimicrobials including the carbapenems.
Using these methods, numerous human and porcine type 078 isolates were indistinguishable and were therefore suggested to have a high-level of genetic relatedness.
Numerous laboratory and patient isolates are frequently found to form rosettes during late trophozoite and schizont stage.
Although these two isolates are distinguished by numerous other synonymous and nonsynonymous polymorphisms (15 ), these organisms are closely related, which has implications for genetic variation based on comparing the two complete genome sequences (14, 29 ).
We recently initiated the characterization of the first laboratory confirmed naturally competent A. baumannii strain, Ab A118, an isolate that unlike other clinical isolates is susceptible to numerous antibiotics, supports replication and stable maintenance of different plasmid replicons and took up fluorophore labeled oligonucleotides (Ramirez et al. 2010, 2011).
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