The enzyme specifically introduces negative supercoiling in a process that must coordinate fuel consumption with DNA cleavage and religation and with numerous conformational changes in both the protein and DNA components of a large nucleoprotein complex.
This encounter complex (ensemble) undergoes numerous conformational changes spread throughout the protein that shuttles the enzyme·substrate complex to a range of conformations where the substrate is tightly bound.
This complex undergoes numerous conformational changes spread throughout the protein that shuttle the enzyme·substrate complex to a range of conformations where the substrate is tightly bound.
Indeed, it might be deceiving to evaluate toxins in isolation given that certain combinations of heavy metals and pesticides were found synergistically provoke numerous histopathological consequences (e.g. conformational changes in alpha-synuclein) and oxidative stress induced neurodegeneration [ 31].
Our model finds numerous applications in modeling of protein motion, conformational changes, protein protein interactions and protein functions, to name just a few.
At a minimum, it is already evident that each of the acyl carrier protein (ACP) domains must undergo large translational, rotational, and conformational changes in conjunction with the numerous successive chain elongation, modification, and translocation events.
Ligand binding to a receptor triggers conformational changes that facilitate receptor dimerization and/or phosphorylation of numerous residues.
Numerous studies indicate that ESCRT-III subunits are activated by intramolecular conformational changes that promote protein-protein interactions (Henne et al., 2012; Lata et al., 2008a; Saksena et al., 2009; Schuh et al., 2015; Shen et al., 2014), but the structural basis for this is obscure.
Protein conformational changes are a constitutive part of the catalytic cycle, and numerous experimental studies, e.g. X-ray [93], NMR [94] and single-molecule experiments [95], have revealed that several conformational transitions occur during the full catalytic cycle.
Although numerous biophysical studies have been devoted to the structure and function of the T domain, a detailed characterization of conformational changes and dynamics at molecular level still has not been achieved.
Conformational changes were monitored by Raman spectroscopy.
