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In addition, we observed numerous cells within the ONH that were immunoreactive for both nestin and Sox9 (Fig. 8f).
Numerous cells within neoplastic emboli showed positive nuclei.
Numerous cells within the surrounding stroma were also positive for [H]thymidine incorporation, indicating that stromal cells as well as epithelial cells were proliferating in the pubertal gland.
As apical constriction during gastrulation is also coordinated in space and time across numerous cells within an epithelial sheet (Martin et al., 2010), it is possible that a similar clutch-like mechanism is allowing such inter-cellular integration of forces.
Similar results were observed with anti-bodies to mitochondrial transcription factor A (TFAM), a nucleoid protein reduced by loss of mtDNA, which showed that numerous cells within cultures A C exhibited reduced TFAM expression (Fig. 3Af h), unlike the homogenous labelling of controls (Fig. 3Ae and Supplementary Material, Fig. S1A).
Immuno-fluorescence with antibodies against anti-DNA, which detect mtDNA nucleoids as small cytoplasmic dots confirmed the presence of numerous cells within cultures A C with mosaic and severe mtDNA depletion, which were not observed in any of the controls (Fig. 3A, top panels).
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In contrast, the basal THy displays a normal tuberal population of Ghrh+ cells at the ArcM core, with less numerous cells dispersed laterally within the ArcW area and caudally under the VM nucleus (ventral VMs); no such cells were found caudal to the IHB (Fig. 12j, k).
The most numerous cell type within the interstitium is the Leydig cell, which is the major source of androgens (Christensen and Mason, 1965; Cooke et al., 1972; Steinberger and Steinberger 1973; van der Molen et al., 1973).
Despite advances in understanding chronic alcohol's detrimental effects on numerous cell types within the lung (Joshi and Guidot, 2007), including the developing alveolar macrophages (AM) (Gauthier et al., 2005, 2009, 2010; Ping et al., 2007), understanding the precise mechanisms by which alcohol exposure alters the function of the AM demands further investigation.
We found numerous cells that expressed Sox2 and Sox9 within the ONH; these cells did not express GFAP or Nkx2.2 (Figs. 4a-d).
Within the plane of the epidermis, numerous cells at similar stages of differentiation are observable, which makes it possible to perform within-sample replicate measurements.
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