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Signal peak areas were converted into numerical intensity values and normalized to internal standards added to each sample and to total signal at each time point.
In the Methods section, we added information on normalization: "Signal peak areas were converted into numerical intensity values and normalized to internal standards added to each sample and to total signal at each time point".
However, the large volume of images produced by such high-throughput screening requires automated image analysis, including the identification and isolation of regions or objects of interest (segmentation) as well as the extraction of numerical intensity and morphology features.
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This method detects genes that are consistently highly ranked in several replicated experiments, independently of their numerical intensities.
It has been found that numerical stress intensity factor results agree with experimental results quite well.
To evaluate the reliability and accuracy of the numerical stress intensity factor results, a full-scale K-joint specimen was tested to failure.
This paper presents a comparison between experimental and numerical stress intensity factors of 3D surface pseudo-semi-elliptical cracks, which are always found at the weld toe of non-load-carrying cruciform welded joints.
Numerical fluorescence intensity values in the images represent means ± SEM of n = 4 images.
While people with mild to moderate dementia are often able to report their pain verbally or use simple visual or numerical pain intensity assessment tools, these options are not feasible for use with people with later stage dementia in whom communication ability is severely impaired [ 7, 8].
Participants also kept a weekly diary of headache number, intensity (numerical rating scale from 1 to 10), and duration during the entire study.
An absolute mean reduction of 3.0 ± 2.1 on a Pain Intensity Numerical Rating Scale (PI-NRS) was noted in the Tramadol Contramid OAD treatment group.
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