Fluorescent images were obtained using a Nikon Eclispse E800 microscope (Nikon, Tokyo, Japan) equipped with a 40 X/0.75 numeric aperture objective lens as well as a Zeiss AxioCam digital camera (Carl Zeiss International, Heidelberg, Germany).
Cultures were washed three times with imaging buffer and transferred to the stage of an inverted Olympus IX70 microscope (Olympus America, Center Valley, PA) equipped with a 60 × 1.25 numeric aperture objective.
The cells were photographed with a 40× objective (numeric aperture 0.95) or a 100× oil objective (numeric aperture 1.25).
Samples were mounted with Mowiol (Calbiochem, La Jolla, CA, USA) and image acquisition was performed with a Leica TCS SP2 Laser Scanning system (Leica, Mannheim, Germany) attached to a DM IRE-2 inverted microscope with a 40× oil objective (numeric aperture 1.25).
Specimens were imaged by confocal fluorescence microscopy using a Zeiss LSM 510 microscope fitted with a Zeiss ×63, numeric aperture 1.4 objective operated in single photon mode, with standard filter sets verified for lack of detectable cross-channel bleed-through and standard (1 Airy disc) pinhole.
The computation of NEFD allowed the characterisation of the shape of silhouettes by objective numeric values.
"Silhouette scores" for assessing the quality of SNP genotyping clusters is convenient for evaluating the quality of SNP genotype assignment, and provides an objective, numeric measure for comparing the performance of SNP assays.
The main advantages of QRT-PCR for the detection of MD are the simplicity with which samples are optimally collected and processed, the transferability of the assays and the objective numeric end point.
In brief, stained semithin sections of the "working series" were scanned at intervals of 2 μm with the image acquisition system "Huge Image" (Knabe et al. 2002; Süss et al. 2002) using the 100× objective (numeric aperture 1.3, image resolution 0.133 μm/pixel = 190.289 dpi).
As we have noted, the objective of numeric criteria is to protect aquatic life uses from anthropogenic nutrient loading to the coastal zone.
