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Numbers of identified reads were normalized by the total numbers of reads in the samples.
To compare expression levels across samples that have differing numbers of reads, it is necessary to normalize the total number of reads to a constant value.
The total numbers of reads are shown in Table 1.
The numbers of reads and TSSs are indicated.
Similar numbers of reads were obtained for each barcoded library.
+ indicates the total numbers of reads obtained after de-multiplexing.
The numbers of reads from nuDNA and the number of reads from mtDNA should reflect the ratio of 2N kM.
Subsequently, counts were normalized by making sample-wise total numbers of reads equal to the median total number of reads for all samples.
These can be estimated from the numbers of reads that span the SV breakpoints and the number of reads that are soft-clipped at these breakpoints, respectively.
For each assembly strategy, an in-house, multithreaded Perl script was used to randomly generate different numbers of reads from the total number of reads.
The numbers of reads in each homologous-protein-group (HPG) were then calculated by summing the numbers of reads aligned to each contig in this group.
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CEO of Professional Science Editing for Scientists @ prosciediting.com