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This is particularly problematic for low copy number transcripts in single cell samples: in the first step of the process, reverse transcription may fail for a small proportion of the original mRNA molecules, which would therefore be eliminated from subsequent amplification and detection.
The real-time principle of reverse transcriptase polymerase chain reaction (RT-PCR) provides a quantitative and reproducible method to detect low copy number transcripts.
The reduced correlation affected high-copy-number transcripts to a lesser extent than it affected low-copy number transcripts (Figure S3).
For low copy number transcripts, the coefficient approached 0.2 as intensity reached background levels.
It is not clear why this proportion of low copy number transcripts is so big in many transcriptomes.
Interestingly, highest number transcripts of copalyl diphosphate synthase (CPS) were present among the mevalonate pathway genes (Fig. 6).
Similar(43)
Comparison of the total number (no). of significantly upregulated (positive numbers) and downregulated (negative numbers) transcripts in the kidney cortex and medulla, liver, lungs, and spleen.
For genes with multiple transcript isoforms, only the first named or numbered transcript was retained.
Total number of transcripts, number of intergenic transcripts, and number of antisense transcripts correspond to the total number of assembled transcripts, the number of assembled transcripts predicted between PlasmoDBv10.0 annotations, and the number of assembled transcripts predicted antisense to PlasmoDBv10.0 annotations.
The large number of transcripts without associated GO terms (7,813 transcripts, ~47%) potentially represent orphan genes.
A gene's "expression profile" denotes the number of transcripts present relative to all other transcripts.
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