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For low copy number replicates which show multiple wells with no amplification, it is possible to determine when a single mutant JAK2 copy has been amplified.
In this case, pollination treatment only was included as a fixed effect and the separate visit number replicates were included in the model as an additional random effect nested in replicate.
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In addition, the ability to take duplicate or higher number replicate measurements may further enhance precision.
Each block contains all the variants so that the block corresponds to a replicate, while the number of replicates equals the number of blocks.
We also use the square of the number of replicates to model the optimal number of replicates.
When the number of replicates finding optimal trees was less than 10%, the number of replicates was increased by 1000.
Overexpression strains of gatA (number of replicates =4) in comparison to the control NW185 PYR A+ (number of replicates =2).
The (< mean) indicates the number of replicates below the mean and the (> mean) indicates the number of replicates above the mean.
Sample size and the number of replicates: sample size was set at 500 individuals, and the number of replicates was 5,000 is all experimental conditions.
The type I error rate was calculated as the number of replicates with p-value <= 0.05 divided by the total number of replicates.
Number or replicates of the central experiment.
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