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All experiments were performed in parallel using equal passage number (passage 3 5) and a similar period in culture.
Cytogenetic analysis of two independent FGF-iPS cell lines revealed a normal karyotype (2n = 40) even after prolonged culture at high passage number (passage 28, 3 months in culture) (Fig. S1C and data not shown).
Single nucleotide polymorphism (SNP) analysis indicated hESCs at early passages had relative genome stability; however, the instability becomes stronger with the increase in passage number (passage >20) [ 14].
All cells were used at a low passage number (passage 4), and subconfluent cultures were used; the cells were plated at 10,000 cells/cm for experiments.
At a higher cell passage number (passage >7), CTCs demonstrated some reversion back to the parental phenotype with respect to ATF3 expression and increased induction in hypoxia (data not shown).
At early passage number (passage 5 or 4), cells expressing lamin A-R220Q are significantly distinguished from those expressing wild-type lamin A. Significant classification was not found, however, between cells expressing lamin A-R220Q at passage 5 and cells expressing WT-lamin A-GFP at passage 9.
Similar(54)
Early-passage-number (passage number 8 15) human umbilical vein endothelial cells (HUVEC) were cultured in fetal calf serum containing endothelial cell growth supplement, epidermal growth factor, heparin, hydrocortisone, and 1 % penicillin/streptomycin in accordance with the manufacturer's instructions (PromoCell GmbH, Heidelberg, Germany).
It is structured as a countdown of numbered passages, starting at 97.
Other applications include custom editions for use in the classroom by inserting questions, comments, or underlining or numbering passages.
To facilitate processing of blinded samples, a coding system using sample number-passage number was applied.
The number of passage times for each airway section was matched in each experiment and it never exceeded 5 passages.
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