The number of spermatozoa in an area of 2 sq mm was counted using the 10 × objective of the microscope after 3 5 min. Estimation of the number of spermatozoa in 1 ml of fluid was done by multiplication of the number counted by 100 000.
This freezing protocol would be suitable for emerging sperm technologies such as sex-preselection of stallion spermatozoa as the sorting process yields only low numbers of spermatozoa in a small volume available for either immediate insemination or cryopreservation.
We showed that exposure to ATZ increases testosterone levels and reduces the number of spermatozoa in epididymis, causing a delay of meiosis.
When insemination was performed once, approximately 18 hours before ovulation and the sows were anesthetized/killed at 4 8 hours after ovulation, there was a tendency towards a larger number of spermatozoa in the UTJ and oviduct among the ACTH sows compared with the control sows [ 10, 73].
ATZ affects recombination during meiosis and many cellular processes, leading to a decrease in the number of spermatozoa in mice.
Caffeine and sperm concentration had an effect on the number of spermatozoa in oocytes and on the polyspermic fertilization rate (P < 0.002).
However, the number of spermatozoa in the ejaculate of dogs given PGF2alpha in the absence of a teaser bitch and in dogs given saline in the presence of a teaser bitch ((556 ± 494 and 600 ± 622) × 106, respectively) were not significantly different from each other or from the other two groups.
Treatment with MCD also increased (P < 0.05) sperm-penetration rate, number of spermatozoa in oocytes, and fertilization efficiency in the caffeine-free fertilization medium.
Reproductive performance, as measured by number of eggs in gravid females and number of spermatozoa in male shrimp was significantly enhanced by both higher concentration of fish oil and astaxanthin.
