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However, being a non-targeted approach, selecting a specific sequence of interest from a vast number of restriction digest products can be intensely challenging and cumbersome10.
A synthetic gene for bovine β-lactoglobulin-variant-A (β-LgA), designed to incorporate a number of restriction sites to form suitable "cassettes" for future site-directed mutagenesis experiments, was constructed as three separate fragments, F1, F2 and F3.
The application of recombinant DNA technology to linkage analysis is revolutionizing the gene mapping field through the availability of an increasing number of restriction fragment length polymorphisms (RFLP).
The resulting hemiboronated extension products are resistant to cleavage by treatment with either DMS and heat or a number of restriction enzymes.
Since these fibers have nanodimensions, they impose a vast number of restriction sites, causing a reduction in tension induced by thermal excitation in the carbon carbon bond.
Briefly, EcoRI digested DNA was additionally digested with HhaI (New England Biolabs) or HpaII (New England Biolabs) and amplified with primers flanking the restriction enzyme sites within the promoter of the specific gene (see Table S1 for primers, enzymes used and number of restriction enzyme sites present within the amplicons).
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Of those who would indicate three-year persistence, 12.3% (10.3%, 14.6%) would indicate the same restrictions at both time points, 20.1% (17.5%, 22.9%) would indicate the same number of restrictions but different areas would be restricted, 35.1% (32.0%, 38.4%) would indicate a lower number of restrictions and 33.2% (29.5%, 35.8%) would indicate a greater number of restrictions at follow-up.
But landmarking brings with it a number of restrictions.
At the time, the company said it was concerned about a number of restrictions in China.
In the early years, a number of restrictions were placed on Jews and Christians.
For example, with regard to access to documents, a number of restrictions are accepted.
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