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The number of latently infected splenocytes can be determined by measuring the number of reactivating splenocytes in the ex vivo reactivation assay and by evaluating the viral genomic load by quantitative PCR.
The consequence for the pathogenesis was a significant reduction in latency amplification, reflected by a reduced extent of splenomegaly, a lower number of reactivating splenocytes and a reduced genomic load during the amplification phase.
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In addition, it is worth mentioning that a relatively high number of reactivated genes are involved in the regulation of posttranslational modifications and of protein synthesis and degradation (in particular the ubiquitin pathway).
Combined treatment of TSA with 5-aza-dC did not cause an increase in the number of reactivated genes which is in accordance with its minor influence on genomic methylation levels.
In the group receiving isoniazid, preventive therapy will decrease the number of reactivated TB cases attributable to isoniazid-susceptible strains but will have less effect on resistant strains, which will increase the proportion of resistant strains among subsequent cases of active TB.
Besides the numbers of reactivated NBs, the sizes of serially homologous clones also differ along the a/p-axis: The total number of progeny cells for each NB decreases from thorax, to A1, to posterior abdomen (supplementary material Fig. S8).
In addition to the spleen weight, we also determined the number of splenocytes reactivating in an ex vivo reactivation assay.
"On any given day, the number of users reactivating their accounts is roughly half of the number of users deactivating their accounts," she said.
However, at days 13 and 17 after infection, less splenocytes reactivated from mice infected with the Delta 40 bp mutant when compared to the parental virus (Fig. 3C and D), whereas the number of splenocytes reactivating from mice infected with the revertant virus was similar to the parental virus at day 17 after infection (Fig. 3D).
At day 10 after infection, a similar number of splenocytes reactivated (Fig. 3B).
The chemotherapeutic action of 5-AZA-CdR may be related not only to the reactivation of specific TSGs, but it may also be dependent on the total number of genes reactivated.
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