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The fraction collector consisted of a tray and a number of flasks.
The suspension of bacilli was received in a number of flasks containing more than 108 cells/mL, averaged and added on 100 mL flasks with sterile weighed quantities of nano-SiO2 (0.01–1.00 g/L), and cultivated during 2 h in the conditions described above.
Decreasing the number of flasks will improve the microbiological safety and traceability and also reduce staff workload and cost.
Once confluence is achieved, trypsin and EDTA are used to disrupt adherence, and MSCs are re-plated within a larger number of flasks [ 30].
The number of stably transduced cells, the number of flasks reseeded and the number of colonies obtained for each flask at 38°C are shown in Additional File 2. The flasks which contained more densely growing/bigger colonies (indicated in red in Additional File 2) were trypsinised, replated and used for extracting genomic DNA when confluent.
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Surfactant emulsification efficiency was done on the basis of percentage transparency and ease of emulsification judged by the number of flask inversions required to yield a homogenous NE.
The volume of media will depend on the total numbers of flasks from which the non-adherent cells were obtained.
To reduce the number of culture flasks used, manufacturing companies such as Nunc and Corning offer large, multilayered culture systems that can fit to usual cell culture incubators.
Although the genetic algorithm can be very efficient for exploration of large variable spaces, it is improbable that a 'global optimum' can be precisely identified because of the relatively small number of shaking flask experiments usually performed.
The same numbers of Erlenmeyer flasks with either 5 ml NS or distilled water were used as negative and positive control groups, respectively.
The same numbers of Erlenmeyer flasks with either 5 ml normal saline or distilled water were used as negative and positive control groups, respectively.
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