To find out the optimized condition for the proposed method, response surface methodology (RSM) was applied for multivariate optimization of effecting parameters namely volume of extractant, number of extraction, pH, and rate of centrifugation.
The variables affecting the extraction step (namely, power of irradiation, irradiation time, extractant volume, extractant composition and number of extraction cycles) have been optimized using experimental design methodology.
Four factors can affect extraction:(A) duration of extraction, (B) maceration time, (C) extract-solvent ratio, and (D) number of extraction.
Finally, the number of extraction cycles necessary for complete extraction was established in two.
Once the method had been optimised, the influence of the number of extraction cycles was studied.
The variables that were studied include temperature, static time, percentage of cyclohexane and the number of extraction cycles.
This is the minimum number of extraction cycles so far reported using quaternary ammonium based eutectic solvents.
Four independent variables such as extraction temperature (°C), ratio of water to raw material, number of extraction, and extraction time (h) were investigated.
Extraction time, extraction temperature, number of extraction and water to raw material ratio were the main factors to influence the yield and purity of the extracted crude polysaccharides.
Three factors were investigated: extraction temperature (60 160 °C), number of extraction cycles (1 3) and time per cycle (2 18 min).
Four independent variables such as extraction temperature (°C), ratio of water to raw material, extraction time (h), and number of extraction (n) were investigated.
