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The amount of Sox17 expression increases with the time of exposure and requires Act, as exposure to Chi alone reduces the levels of expression and the number of expressing cells (Fig. 3A).
When levels of Rho1 or Zip were reduced in RhoGEF2-expressing clones, msn-lacZ expression was still detectable in the lateral region clones, but was significantly reduced relative to RhoGEF2 expression alone, reflecting a decrease in either the number of expressing cells or in the level of expression (arrows, Fig. 5B-Bii,C-Cii; quantified in 5F).
The expression profile at the irreversible stage shows a unique pattern of a wide range of expression levels and an increased number of expressing genes, especially the cancer-related genes.
The reduction was incomplete, however, leaving open the possibility that reduced expression of mutant protein in muscle or a reduced number of expressing fibers is sufficient to cause the motor neuron disease phenotype.
The low number of expressing cells was also reflected in the low level of mRNA expression detected by TaqMan analysis.
The number of expressing cells then decreases until, in late embryos and all post-hatching stages, expression was seen only in one pair of head nerves.
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The number of expressed unigenes and distribution of expression levels in each tissue are shown in Figure 3.
For gene expression analysis, the number of expressed tags was counted and then normalized to TPM.
The number of expressed genes as well the gene expression measurements differ significantly between different isolation techniques.
The number of expressed genes however also drops considerably with increasing expression thresholds.
Absolute levels of gene expression, normalized to plasmid standards, indicated large differences in number of expressed transcripts among genes.
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