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By attaching a number of detergent molecules to traditional enzymes, scientists have managed to create an enzyme liquid (without water) that still allows the folded proteins to catalyze the reactions of life.
The Nirma group of industries, which runs a large number of detergent and chemical industrial units, has its corporate headquarters in the city.
This is defined as the average number of detergent monomers in a micelle.
The protein Smr, staphylococcal multidrug resistance pump, binds substrate in isotropic bicelles, but shows only reduced or unspecific binding in a number of detergent systems.
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Enterokinase and factor Xa were only affected by a small number of detergents, making them good choices as well.
The conclusions reached by this discussion would not only enable MP scientists to rationally select a set of detergent candidates among a large number of detergents but also provide detergent inventors with useful guidelines in designing novel amphipathic systems.
The P3 fraction from the brains of PrP knockout mice (Prnp0/0) showed a similar pattern of bands (with the exception of the PrP-specific bands) (Fig. 1C, lane 4), confirming that the P3 pellet contained a number of detergent-insoluble proteins besides PrP.
Only a limited number of detergents are suitable for D-CF expression or for resolubilization after P-CF expression.
Raft-organisation was subsequently studied using a number of detergent-free experimental approaches.
A different number of detergent-enzymatic cycles, 3 and 7 cycles, respectively, were performed.
To confirm cysts formation, the pellet was resuspended, in 1 mL of 0.2% w/v Triton X-100 for 10 min, and the number of detergent-resistant cysts was determined using a haemocytometer.
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